Published Paper

L’Occitane – Colonization of a 3D Skin Model

3D skin models are getting closer to the reality of cutaneous physiology. They can include multiple cell types, from healthy or pathological skin or be submitted to various stresses mimicking the skin’s environment.

But most of these models are sterile. In recent years, skin microbiota has emerged as a key player in skin health, preventing pathogens proliferation, educating the immune system and maintaining barrier integrity. The first skin models colonized with bacteria have focused on unique bacterial species. But the study of skin diseases (such as atopic dermatitis) revealed that microbiota’s diversity is key to skin health. We thus developed a model that reproduces the complexity of the skin’s ecosystem with an uncultured skin microbiota and compared it to the use of a unique commensal bacteria.

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