Mass spectrometry imaging (MSI) is a powerful tool for the study of intact tissue sections. Here, its application to the study of the distribution of lipids in sections of reconstructed living skin equivalents during their development and maturation is described.
Living skin equivalent (LSE) samples were obtained at 14 days development, resuspended in maintenance medium and incubated for 24h after delivery. The medium was then changed, the LSE re-incubated and samples taken at 4, 6 and 24h time points. Mass spectra and mass spectral images were recorded from 12μm sections of the LSE taken at each time point for comparison using matrix assisted laser desorpt ionionisation mass spectrometry.